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Hemoglobin epsilon antibody [HL2457]

Anti-Hemoglobin epsilon antibody [HL2457] used in IHC (Paraffin sections) (IHC-P). GTX638775
Anti-Hemoglobin epsilon antibody [HL2457] used in IHC (Paraffin sections) (IHC-P). GTX638775
Anti-Hemoglobin epsilon antibody [HL2457] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638775
Anti-Hemoglobin epsilon antibody [HL2457] used in Western Blot (WB). GTX638775

Cat. No. GTX638775

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2457

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide of human Hemoglobin epsilon.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hemoglobin subunit epsilon 1 , HBE

Background

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hemoglobin epsilon antibody [HL2457] used in IHC (Paraffin sections) (IHC-P). GTX638775

GTX638775 IHC-P Image

Hemoglobin epsilon antibody [HL2457] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hemoglobin epsilon antibody [HL2457] used in IHC (Paraffin sections) (IHC-P). GTX638775

GTX638775 IHC-P Image

Hemoglobin epsilon antibody [HL2457] detects secreted Hemoglobin epsilon protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hemoglobin epsilon antibody [HL2457] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638775

GTX638775 ICC/IF Image

Hemoglobin epsilon antibody [HL2457] detects Hemoglobin epsilon protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hemoglobin epsilon stained by Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Hemoglobin epsilon antibody [HL2457] used in Western Blot (WB). GTX638775

GTX638775 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [HL2457] (GTX638775) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations