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Hemoglobin epsilon antibody [N1N2], N-term

Orthogonal
GTX108396_39848_20160331_WB_M_CFA_19011507_963.jpg
GTX108396_39848_20160331_WB_H_plasma_19011507_109.jpg
GTX108396_39848_20160414_WB_R_spleen_19011507_415.jpg

Cat No. GTX108396

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

human plasma , Mouse Leukocyte , mouse leukocyte treated with CFA , rat spleen , *Human first trimester placentas

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Hemoglobin epsilon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hemoglobin Subunit Epsilon 1,Hbe,Hbe1

Background

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008]

Database

DATA IMAGES
GTX108396_39848_20160331_WB_M_CFA_19011507_963.jpg

GTX108396 WB Image

Orthogonal

Untreated (–) and treated (+) mouse tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:500. (CFA: Complete Freunds adjuvant)

GTX108396_39848_20160331_WB_H_plasma_19011507_109.jpg

GTX108396 WB Image

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:500.

GTX108396_39848_20160414_WB_R_spleen_19011507_415.jpg

GTX108396 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:1000.

REVIEW

Hemoglobin epsilon antibody [N1N2], N-term Cat No. GTX108396

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Aug-2013
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : First trimester placentas
Blocking : 2.5% Horse serum, 25°C, 0.5Hr
Fixation : 4% PFA overnight and 70% EtOH for storage
Permeabilization /Antigen retrieval : 0.5% Tween in PBS/A.Menarini Access Super,pH 9.5
Primary Antibody : 1:8000, 25°C, 0.5Hr
Figure Note : Remove background HRP using 3% hydrogen peroxide
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169