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Hemoglobin epsilon antibody [N1N2], N-term

Anti-Hemoglobin epsilon antibody [N1N2], N-term used in Western Blot (WB). GTX108396
Anti-Hemoglobin epsilon antibody [N1N2], N-term used in Western Blot (WB). GTX108396
Anti-Hemoglobin epsilon antibody [N1N2], N-term used in Western Blot (WB). GTX108396

Cat No. GTX108396

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

16 kDa. ( Note )

Positive Control

human plasma , Mouse Leukocyte , mouse leukocyte treated with CFA , rat spleen , *Human first trimester placentas

Predict Reactivity

Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.41 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Hemoglobin epsilon. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037151

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hemoglobin subunit epsilon 1 , HBE

Background

The epsilon globin gene (HBE) is normally expressed in the embryonic yolk sac: two epsilon chains together with two zeta chains (an alpha-like globin) constitute the embryonic hemoglobin Hb Gower I; two epsilon chains together with two alpha chains form the embryonic Hb Gower II. Both of these embryonic hemoglobins are normally supplanted by fetal, and later, adult hemoglobin. The five beta-like globin genes are found within a 45 kb cluster on chromosome 11 in the following order: 5'-epsilon - G-gamma - A-gamma - delta - beta-3' [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Hemoglobin epsilon antibody [N1N2], N-term used in Western Blot (WB). GTX108396

GTX108396 WB Image

Untreated (–) and treated (+) mouse tissue extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:500. (CFA: Complete Freunds adjuvant)

Anti-Hemoglobin epsilon antibody [N1N2], N-term used in Western Blot (WB). GTX108396

GTX108396 WB Image

Human tissue extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:500.

Anti-Hemoglobin epsilon antibody [N1N2], N-term used in Western Blot (WB). GTX108396

GTX108396 WB Image

Rat tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Hemoglobin epsilon antibody [N1N2], N-term (GTX108396) diluted at 1:1000.

REFERENCE

There are currently no references for Hemoglobin epsilon antibody [N1N2], N-term (GTX108396). Be the first to share your publications with this product.

REVIEW

Hemoglobin epsilon antibody [N1N2], N-term Cat No. GTX108396

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 20-Aug-2013
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : First trimester placentas
Blocking : 2.5% Horse serum, 25°C, 0.5Hr
Fixation : 4% PFA overnight and 70% EtOH for storage
Permeabilization /Antigen retrieval : 0.5% Tween in PBS/A.Menarini Access Super,pH 9.5
Primary Antibody : 1:8000, 25°C, 0.5Hr
Figure Note : Remove background HRP using 3% hydrogen peroxide
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169