Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:100-1:200 (0.5-1μg labels ~10⁵ cells) |
1:100-1:500 |
1:50-1:100 |
Not tested in other applications.
Product Note
Recognizes an epitope present in many types of human heparan sulfate. The epitope includes N-sulfated glucosamine residues that are critical for the reactivity of the antibody. Does not react with hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate or DNA. Reactivity with most heparan sulfates is nearly completely abolished after treatment of the glycosaminoglycan with bacterial heparitinase (Flavobacterium heparinum, EC 4.2.2.8).
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Liposome-incorporated membrane heparan sulfate proteoglycan from human fetal lung fibroblasts.
Purification
Purified by tangential ultrafiltration
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.