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Heparanase 1 antibody

Orthogonal
Anti-Heparanase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32650
Anti-Heparanase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32650
Anti-Heparanase 1 antibody used in Western Blot (WB). GTX32650
Anti-Heparanase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32650
Anti-Heparanase 1 antibody used in Western Blot (WB). GTX32650

Cat. No. GTX32650

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Heparanase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

61 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 36-275 of human HPSE (NP_001092010.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887671

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heparanase , HPA , HPA1 , HPR1 , HPSE1 , HSE1

Cellular Localization

Secreted,Nucleus

Background

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-Heparanase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32650

GTX32650 ICC/IF Image

ICC/IF analysis of BALB-3T3 cells using GTX32650 Heparanase 1 antibody.
Blue : DAPI

Anti-Heparanase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32650

GTX32650 ICC/IF Image

ICC/IF analysis of C6 cells using GTX32650 Heparanase 1 antibody.
Blue : DAPI

Anti-Heparanase 1 antibody used in Western Blot (WB). GTX32650

GTX32650 WB Image

WB analysis of various sample lysates using GTX32650 Heparanase 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Heparanase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32650

GTX32650 ICC/IF Image

Orthogonal

The data was published in the journal Sci Rep in 2019. PMID: 31537875

Anti-Heparanase 1 antibody used in Western Blot (WB). GTX32650

GTX32650 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2019. PMID: 31537875

REFERENCE

Application Reference

Kiyan Y et al. Sci Rep 2019; 9 (1) : 13591 Heparanase-2 protects from LPS-mediated endothelial injury by inhibiting TLR4 signalling.
Application : WB
Reactivity : Human
Yulia Kiyan et al. bioRxiv 2019; (Epub) Heparanase-2 protects from endothelial injury by inhibiting TLR4 signaling.
Application : WB
Reactivity : Human
Chen HR et al. PLoS Pathog 2018; 14 (4) : e1007033 Macrophage migration inhibitory factor is critical for dengue NS1-induced endothelial glycocalyx degradation and hyperpermeability.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399