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Hepatitis B Virus Core Antigen antibody [C1]

Anti-Hepatitis B Virus Core Antigen antibody [C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40523

Cat No. GTX40523

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 C1

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, ELISA, IHC, IM

Reactivity

 Hepatitis B virus
Package
100 μg ($379)
See all Hepatitis B Virus Core Antigen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
IM Assay dependent
Not tested in other applications.

Specificity/Sensitivity

Hepatitis B Virus Core Antigen (a.a. 70-80)

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Hepatitis B virus

Immunogen

Recombinant HBcAg core antigen (ayw).

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

Anti-Hepatitis B Virus Core Antigen antibody [C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX40523

GTX40523 ICC/IF Image

Hepatitis B core antigen antibody [C1] detects Hepatitis B core antigen protein by immunofluorescent analysis.
Samples: Huh-7 cells mock (left) and transfected with HBV (adw) (right) were fixed in 4% paraformaldehyde for 10 min.
Green: Hepatitis B core antigen protein stained by Hepatitis B core antigen antibody [C1] (GTX40523) diluted at 1:300.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Dorobantu C et al. J Virol 2011; 85 (24) : 13373-83 Cholesterol depletion of hepatoma cells impairs hepatitis B virus envelopment by altering the topology of the large envelope protein.
Application : IP

Clone Reference

Liu B et al. Autophagy 2014; 10 (3):416-30 Hepatitis B virus X protein inhibits autophagic degradation by impairing lysosomal maturation.
Application : WB
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SDS
PBS.pdf
Package List Price ($)
$ 379