*Optimal dilutions/concentrations should be determined by the researcher.
Application
Recommended Dilution
WB
Assay dependent
ICC/IF
1:100-1:1000
IP
Assay dependent
ELISA
Assay dependent
IHC
Assay dependent
Not tested in other applications.
Product Note
HBcAg core antigen. Produced against recombinant HBcAg core antigen (ayw). Reacts with synthetic HBcpeptides.
PROPERTIES
Form
Liquid
Buffer
Ascites diluted with PBS pH7.4
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Hepatitis B virus
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_423351
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Hepatitis B core antigen antibody [10E11] detects Hepatitis B core antigen protein by immunofluorescent analysis. Samples: Huh-7 cells mock (left) and transfected with HBV (adw) (right) were fixed in 4% paraformaldehyde for 10 min. Green: Hepatitis B core antigen protein stained by Hepatitis B core antigen antibody [10E11] (GTX18686) diluted at 1:300. Blue: Hoechst 33342 staining.
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