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Hepatitis B virus Core Antigen antibody [10E11]

Anti-Hepatitis B virus Core Antigen antibody [10E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18686

Cat No. GTX18686

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10E11

Isotype

 IgG2a

Application

 WB, ICC/IF, IP, ELISA, IHC

Reactivity

 Hepatitis B virus
Package
100 μg ($369)
See all Hepatitis B virus Core Antigen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF 1:100-1:1000
IP Assay dependent
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

HBcAg core antigen. Produced against recombinant HBcAg core antigen (ayw). Reacts with synthetic HBcpeptides.

PROPERTIES

Form

Liquid

Buffer

Ascites diluted with PBS pH 7.4, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Hepatitis B virus

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_423351

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Hepatitis B Virus Core , HBV Core

Research Area

DATA IMAGES

Anti-Hepatitis B virus Core Antigen antibody [10E11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX18686

GTX18686 ICC/IF Image

Hepatitis B core antigen antibody [10E11] detects Hepatitis B core antigen protein by immunofluorescent analysis.
Samples: Huh-7 cells mock (left) and transfected with HBV (adw) (right) were fixed in 4% paraformaldehyde for 10 min.
Green: Hepatitis B core antigen protein stained by Hepatitis B core antigen antibody [10E11] (GTX18686) diluted at 1:300.
Blue: Hoechst 33342 staining.

REFERENCE

Application Reference

Dorobantu C et al. J Virol 2011; 85 (24) : 13373-83 Cholesterol depletion of hepatoma cells impairs hepatitis B virus envelopment by altering the topology of the large envelope protein.
Application : IP
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 369