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Hepatitis B virus X protein antibody [X36C]

Anti-Hepatitis B virus X protein antibody [X36C] used in Western Blot (WB). GTX22741

Cat. No. GTX22741

Host

Mouse

Clonality

Monoclonal

Clone Name

X36C

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, IP, ELISA, ChIP assay, IHC

Reactivity

Hepatitis B virus
Package
100 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 μg/ml
ICC/IF Assay dependent
IHC-P Assay dependent
IP Assay dependent
ELISA Assay dependent
ChIP assay Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

This antibody is not capable of immunoprecipitating HBx complexed with HBx interacting protein (XIP).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Hepatitis B virus

Immunogen

Baculovirus expressed recombinant HBx.

Purification

Purified by HPLC

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Hepatitis B Virus X antigen , HBV X antigen

Background

The hepatitis B virus is an important cause of acute and chronic liver disease. It is also the most common form of parenterally transmitted viral hepatitis. The virus is estimated to affect some 350 million people worldwide. Immunization against hepatitis B is an important component of preventative medicine and is offered to all children in approximately 30 countries. Hepatitis B virus (HBV) belongs to the Hepadnaviridae family and infection with HBV is usually more serious than infection with hepatitis A virus. In some individuals the infection is not cleared and followed by persistent intracellular viral carriage that can lead to the development of hepatocelular carcinomas. There are currently 400 million HBV carriers worldwide making HBV one of the most common human pathogens.

Research Area

DATA IMAGES

Anti-Hepatitis B virus X protein antibody [X36C] used in Western Blot (WB). GTX22741

GTX22741 WB Image

Non-transfected (–) and transfected (+) Huh7 whole cell extracts (25 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis B Virus X Protein antibody [X36C] (GTX22741) diluted at 1:1000.

REFERENCE

REVIEW

Hepatitis B virus X protein antibody [X36C] Cat. No. GTX22741

Rating ( Average 3 based on 1 users reviews)
Application
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 12-Jan-2016
Score :
Application Tested : WB
Sample Species : HBV
Sample : HBV infected Huh 7 cells
Amount used : 25μg
Blocking : 4% milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Huh 7 cells, Lane 2: HBV infected Huh 7 cells
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399