ELISA: Use at an assay dependent dilution. GTX18700 was tested using indirect ELISA method. The testing plates were coated with HCV non-structural protein (NS), capsid protein (C) and the mixture of NS and C separately. The neat culture supernatant of hybridoma showed a strong reaction with HCV capsid protein (OD492 value > 2.0) and mixture of NS and C (OD492 value >1.5), and have no reaction with HCV non-structural protein (OD492 value < 0.03). Is unsuitable for WB. Optimal dilutions/concentrations should be determined by the end user.
GTX18700 detects recombinant capsid protein C (CPC), envelope protein M (EPM) core; amino acids 1-142) and synthetic capsid protein C (amino acids 1-61). GTX18700 recognizes different antigenic determinants of Hepatitis C Core Antigen.
0.01M PBS pH7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Hepatitis C virus
Synthetic peptides derived from HCV capsid protein.
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Hepatitis C Virus Core , HCV Core
Hepatitis C virus (HCV) is the primary cause of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. The 21 kDa core protein of HCV is well conserved among different HCV genotypes and may suppress hepatitis B virus replication in a phosphorylation dependent manner.
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