ELISA: Use at an assay dependent dilution. Detects human Hepatitis C Virus core protein but not HCV non-structural protein. WB: Use at a concentration of 0.1 - 0.5 μg/ml. This allows visualization of 0.1 ug/lane of recombinant CPC+EPM (core), 0.5 ug/lane synthetic CPC, and 0.1 ug/lane recombinant chimeric HCV polyprotein. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user.
GTX18728 detects recombinant capsid protein C and envelope protein M (1-142 aa), synthetic CPC (1-61 aa) and recombinant chimeric HCV polyprotein (60kDa). No cross reactivity is observed with recombinant NS3 protein (125-147 aa), synthetic NS3 protein (1378-1458 aa) and synthetic NS4a protein (1689-1735 aa). GTX18728 recognizes different antigenic determinants of HCV capsid protein. GTX18728 can be used to detect in-vitro translated HCV core protein.
0.01M PBS pH7.0
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Hepatitis C virus
Synthetic peptide (Human).
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Hepatitis C Virus Core , HCV Core
Hepatitis C virus (HCV) is the primary cause of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. The 21 kDa core protein of HCV is well conserved among different HCV genotypes and may suppress hepatitis B virus replication in a phosphorylation dependent manner.