ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 1 μg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user. GTX18664 will allow visualization of 100 ng/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3 Protein. No reaction is seen with synthetic NS-3.
GTX18664 reacts with recombinant NS-3 (1252aa-1477aa), synthetic NS-3 (1378aa-1458aa), and recombinant chimeric HCV polyprotein (60 kDa). No cross reaction can be seen with recombinant CPC* + EPM** (core) (amino acids 1-142), synthetic CPC* (amino acids 1-61), and synthetic NS-4 protein (amino acids 1689-1735).
0.01M PBS pH7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Hepatitis C virus
Purified recombinant chimeric HCV polyprotein (555 amino acids)
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Hepatitis C Virus Serine protease NS3 , Hepatitis C Virus NS3 , HCV Serine protease NS3 , HCV NS3
Cell Membrane, Cytoplasmic and Plasma membrane
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.