ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 60 kDa. GTX18663 will allow visualization of 0.1 ug/lane of synthetic NS-3 protein. Optimal dilutions/concentrations should be determined by the end user.
Product Note
Specific for synthetic NS-3 Protein (amino acids 1378-1458). No cross reaction with HCV capsid region and other non-structural regions.
PROPERTIES
Form
Liquid
Buffer
0.01M PBS pH7.0
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Hepatitis C virus
Immunogen
A highly antigenic polypeptide consisting of essential sequences of at least 60 residues in length, which were selected from genes encoding the NS-3 region of Chinese HCV strains.
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_423333
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Hepatitis C Virus Serine protease NS3 , Hepatitis C Virus NS3 , HCV Serine protease NS3 , HCV NS3
Cellular Localization
Cell Membrane, Cytoplasmic and Plasma membrane
Background
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
