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Hepatitis C virus NS3 protein antibody [328]

Cat. No. GTX18667

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 328

Isotype

 IgG1

Application

 WB, ELISA

Reactivity

 Hepatitis C virus
Package
250 μg ($399)
See all Hepatitis C virus NS3 protein products

APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. WB: Use at a concentration of 0.1 - 1 μg/ml. Predicted molecular weight: 60 kDa. Optimal dilutions/concentrations should be determined by the end user. GTX18667 will allow visualization of 0.1 ug/lane of both recombinant chimeric HCV polyprotein and recombinant NS-3.

Product Note

GTX18667 reacts with recombinant NS-3 (amino acids 1252-1477) and recombinant chimeric HCV polyprotein (60 kDa). No cross reaction can be seen with recombinant CPC* + EPM** (core) (amino acids 1-142), synthetic CPC* (amino acids 1-61), synthetic NS-3 (amino acids 1378-1458) and synthetic NS-4a Protein (amino acids 1689-1735).

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.2

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Hepatitis C virus

Immunogen

Purified recombinant chimeric HCV Polyprotein (555 a.a)

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Hepatitis C Virus Serine protease NS3 , Hepatitis C Virus NS3 , HCV Serine protease NS3 , HCV NS3

Cellular Localization

Cell Membrane, Cytoplasmic and Plasma membrane

Background

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations