For ELISA: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Not tesed in other applications. Optimal dilutions/concentrations should be determined by the researcher.
Reacts with Recombinant NS-3 (1252a.a.-1477a.a) and Recombinant Chimeric HCV Polyprotein (60 kDa). No cross-reactivity can be seen with recombinant CPC* + EPM** (core) (1a.a.-142a.a.), synthetic CPC* (1a.a.-61a.a.), synthetic NS-3 Protein (1378a.a.-1458a.a) and synthetic NS-4a Protein (1689a.a.-1735a.a)
0.01M PBS pH7.2
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Hepatitis C virus
Purified recombinant chimeric HCV polyprotein (555 amino acids).
Protein G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Hepatitis C Virus Serine protease NS3 , Hepatitis C Virus NS3 , HCV Serine protease NS3 , HCV NS3
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.