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Hepatitis C virus NS4A protein antibody [5D4/10E7]

Cat No. GTX19048

Host

Mouse

Clonality

Monoclonal

Clone Name

5D4/10E7

Isotype

IgG1

Application

WB, IHC-P, ELISA

Reactivity

Hepatitis C virus
Package
250 μg ($379)

APPLICATION

Application Note

ELISA: Use at an assay dependent dilution. IHC-P: The dilution fold should be from 1:100 to 1:1000. WB: Use at a concentration of 0.5 μg/ml to detect 0.5 μg/lane of synthetic NS4 peptide and 0.1 μg/lane recombinant chimeric HCV polyprotein. Optimal dilutions/concentrations should be determined by the end user.

Product Note

This antibody reacts with synthetic NS4a Protein, and recombinant Chimeric HCV Polyprotein. No cross-reaction with HCV capsid region and other non-structure regions.

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.0

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Hepatitis C virus

Immunogen

Synthetic peptide (Human) in which overlapping sequences of at least 90 residues in length were selected from the immunodominant NS4 regions of Chinese HCV strains.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Hepatitis C Virus Non-structural protein 4A , Hepatitis C Virus NS4A , HCV Non-structural protein 4A , HCV NS4A

Cellular Localization

RNA virus

Background

HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non-structural protein necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 379
Custom Conjugations