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Hepatitis C virus NS4A protein antibody [S4-13]

Anti-Hepatitis C virus NS4A protein antibody [S4-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64116
Anti-Hepatitis C virus NS4A protein antibody [S4-13] used in Western Blot (WB). GTX64116

Cat No. GTX64116

Host

Mouse

Clonality

Monoclonal

Clone Name

S4-13

Isotype

IgG2b

Application

WB, ICC/IF, ELISA

Reactivity

Hepatitis C virus
Package
100 μg ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

Specific to human HCV NS4a protein of genotype 1b. It does not react with genotypes 1a, and 2a.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Hepatitis C virus

Immunogen

A region of NS4 protein of HCV genotype 1b expressed in E.coli. The epitope of this antibody was mapped to the N-terminal region of the NS4 protein (NS4a).

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Hepatitis C Virus Non-structural protein 4A , Hepatitis C Virus NS4A , HCV Non-structural protein 4A , HCV NS4A

Research Area

DATA IMAGES

Anti-Hepatitis C virus NS4A protein antibody [S4-13] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64116

GTX64116 ICC/IF Image

Detection of HCV NS4a protein by immunofluorescence antibody staining. Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and HCV NS4a protein was detected by indirect immunofluorescence staining using this antibody.

Anti-Hepatitis C virus NS4A protein antibody [S4-13] used in Western Blot (WB). GTX64116

GTX64116 WB Image

Western blotting of HCV NS4A protein. Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using anti-NS4a antibody. The protein detected with this antibody is 6 kD. This small NS4 protein (NS4a) was produced from the N-terminal region of the NS4 protein.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 379