GeneTex
  • Country / Location Selection

United States (US)

Hepatitis D virus Large delta protein antibody

Anti-Hepatitis D virus Large delta protein antibody used in Western Blot (WB). GTX135574
Anti-Hepatitis D virus Large delta protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135574
Anti-Hepatitis D virus Large delta protein antibody used in Western Blot (WB). GTX135574
Anti-Hepatitis D virus Large delta protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135574

Cat. No. GTX135574

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P (cell pellet)

Reactivity

Hepatitis D virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
Not tested in other applications.

Product Note

Based on both internal and collaborating lab testing, this antibody is specific for HDV large delta antigen and does not detect HDV small delta antigen.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Hepatitis D virus

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Hepatitis D virus Large delta protein (HDV genotype I (strain D380)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887499

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Hepatitis D virus Large delta protein antibody used in Western Blot (WB). GTX135574

GTX135574 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis D virus Large delta protein antibody (GTX135574) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hepatitis D virus Large delta protein antibody used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX135574

GTX135574 IHC-P (cell pellet) Image

Hepatitis D virus Large delta protein antibody detects Hepatitis D virus Large delta protein at cytoplasm by immunohistochemical analysis.
Sample:Paraffin-embedded mock and Hepatitis D virus Large delta protein transfected 293T cell pellet.
Green: Hepatitis D virus Large delta protein stained by Hepatitis D virus Large delta protein antibody (GTX135574) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hepatitis D virus Large delta protein antibody used in Western Blot (WB). GTX135574

GTX135574 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Hepatitis D virus Large delta protein antibody (GTX135574) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hepatitis D virus Large delta protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX135574

GTX135574 ICC/IF Image

Hepatitis D virus Large delta protein antibody detects Hepatitis D virus Large delta protein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Hepatitis D virus Large delta protein stained by Hepatitis D virus Large delta protein antibody (GTX135574) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

There are currently no references for Hepatitis D virus Large delta protein antibody (GTX135574). Be the first to share your publications with this product.

REVIEW

Hepatitis D virus Large delta protein antibody Cat. No. GTX135574

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-Apr-2021
Score :
Application Tested : WB
Sample Species : HDV
Primary Antibody : 1:1000, °C, Hr
Figure Note : *Data provided by Dr. Fuxuan Wang from Dr. Jinhong Chang's lab at the Baruch S. Blumberg Institute (BSBI).
pDL445: express HDAg large antigen
pDL444: express HDAg small antigen
pSVLD3: trimer of HDV cDNA, express HDAg small and large as result of genome replication
pSV45H: express HBV surface antigens
PSVLD3+pSV45H: initiate HDV genome replication and secret virions using HBV surface antigens as envelop
Package List Price ($)
$ 399
$ 169