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Her2 / ErbB2 antibody [6C2]

Anti-Her2 / ErbB2 antibody [6C2] used in Flow cytometry (FACS). GTX82764
Anti-Her2 / ErbB2 antibody [6C2] used in Western Blot (WB). GTX82764
Anti-Her2 / ErbB2 antibody [6C2] used in IHC (Paraffin sections) (IHC-P). GTX82764

Cat. No. GTX82764

1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6C2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Her2 / ErbB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF Assay dependent
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ERBB2(aa750-987) expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1

Cellular Localization

Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 antibody [6C2] used in Flow cytometry (FACS). GTX82764

GTX82764 FACS Image

FACS analysis of MCF-7 cells using GTX82764 Her2 / ErbB2 antibody [6C2].
Green : Her2 / ErbB2
Purple : negative control

Anti-Her2 / ErbB2 antibody [6C2] used in Western Blot (WB). GTX82764

GTX82764 WB Image

WB analysis of SKBR3 (1) and MCF-7 (2) cell lysate using GTX82764 Her2 / ErbB2 antibody [6C2].

Anti-Her2 / ErbB2 antibody [6C2] used in IHC (Paraffin sections) (IHC-P). GTX82764

GTX82764 IHC-P Image

IHC-P analysis of galactophore tumour using GTX82764 Her2 / ErbB2 antibody [6C2].

REFERENCE

There are currently no references for Her2 / ErbB2 antibody [6C2] (GTX82764). Be the first to share your publications with this product.
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REVIEW

Her2 / ErbB2 antibody [6C2] Cat. No. GTX82764

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Her2 / ErbB2 antibody [6C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82764
Date : Anonymous submitted on 29-Sep-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : NCI-N87 cells, CFPAC-1 cells, CAPAN-1 cells, and MCF-7 cells
Blocking : 0.5% BSA + 1 μg/mL Goat polyclonal antibody in PBS, 25°C, 0.5Hr
Fixation : 4% PFA in PBS, RT, 10 mins
Permeabilization /Antigen retrieval : Dako Target Retrieval Solution, pH6.0, 10 mins, 120°C
Primary Antibody : 1:400, 4°C, 18Hr
Figure Note :
Endogenous blocking : Dako REAL Peroxidase-Blocking Solution (Agilent, Catalog No.S2023), RT, 10 mins
Detection system : Dual Link System-HRP (Dako，Catalog No.K4063)
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399