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Her2 / ErbB2 antibody [HRB2/451]

Anti-Her2 / ErbB2 antibody [HRB2/451] used in IHC (Paraffin sections) (IHC-P). GTX34780
Anti-Her2 / ErbB2 antibody [HRB2/451] used in IHC (Paraffin sections) (IHC-P). GTX34780

Cat No. GTX34780

Host

Mouse

Clonality

Monoclonal

Clone Name

HRB2/451

Isotype

IgG1

Application

ICC/IF, IHC-P

Reactivity

Human
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Specificity/Sensitivity

This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

PROPERTIES

Form

Liquid

Buffer

10mM PBS with 0.05% BSA, 0.05% azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml

Antigen Species

Human

Immunogen

Recombinant human HER-2 protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Erb-B2 Receptor Tyrosine Kinase 2 , Cd340 , Her-2 , Her-2/Neu , Her2 , Mln 19 , Neu , Ngl , Tkr1 , Erbb2

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 antibody [HRB2/451] used in IHC (Paraffin sections) (IHC-P). GTX34780

GTX34780 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HER-2 Monoclonal Antibody (HRB2/451).

Anti-Her2 / ErbB2 antibody [HRB2/451] used in IHC (Paraffin sections) (IHC-P). GTX34780

GTX34780 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HER-2 Monoclonal Antibody (HRB2/451).

REFERENCE

There are currently no references for Her2 / ErbB2 antibody [HRB2/451] (GTX34780). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Her2 / ErbB2 antibody [HRB2/451] (GTX34780). Be the first to share your experience with this product.
Package List Price ($)
$ 429