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Her2 / ErbB2 antibody [HRB2/451]

Anti-Her2 / ErbB2 antibody [HRB2/451] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34780
Anti-Her2 / ErbB2 antibody [HRB2/451] used in Flow cytometry (FACS). GTX34780
Anti-Her2 / ErbB2 antibody [HRB2/451] used in Flow cytometry (FACS). GTX34780
Anti-Her2 / ErbB2 antibody [HRB2/451] used in Protein Array (Protein Array). GTX34780

Cat No. GTX34780

Host

Mouse

Clonality

Monoclonal

Clone Name

HRB2/451

Isotype

IgG1

Application

ICC/IF, FACS, ELISA, Protein Array

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-4μg/ml
FACS 1-2μg/10⁶ cells
ELISA Assay dependent
Protein Array Assay dependent

Note :

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Specificity/Sensitivity

This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human HER-2 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD340 , ERBB2 , HER2 , HER2/neu , MLN 19 , NEU , NGL , TKR1 , erbb2 receptor tyrosine kinase 2 , Her2 , ErbB2 , Neu , EGFR2 , erb-b2 receptor tyrosine kinase 2

Cellular Localization

Cell membrane,Cytoplasm,Perinuclear region,Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 antibody [HRB2/451] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34780

GTX34780 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34780 Her2 / ErbB2 antibody [HRB2/451].
Green : Primary antibody
Red : nucleus

Anti-Her2 / ErbB2 antibody [HRB2/451] used in Flow cytometry (FACS). GTX34780

GTX34780 FACS Image

FACS analysis of trypsinized MCF-7 cells using GTX34780 Her2 / ErbB2 antibody [HRB2/451].
Blue : Primary antibody
Red : Isotype control

Anti-Her2 / ErbB2 antibody [HRB2/451] used in Flow cytometry (FACS). GTX34780

GTX34780 FACS Image

FACS analysis of trypsinized SK-BR3 cells using GTX34780 Her2 / ErbB2 antibody [HRB2/451].
Blue : Primary antibody
Red : Isotype control

Anti-Her2 / ErbB2 antibody [HRB2/451] used in Protein Array (Protein Array). GTX34780

GTX34780 Protein Array Image

Analysis of Protein Array containing more than 19,000 full-length human proteins using HER-2 Monospecific Mouse Monoclonal Antibody (HRB2/451). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf