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Her2 / ErbB2 antibody [RM228]

Anti-Her2 / ErbB2 antibody [RM228] used in Western Blot (WB). GTX33616
Anti-Her2 / ErbB2 antibody [RM228] used in Immunohistochemistry (IHC). GTX33616

Cat No. GTX33616

Host Rabbit
Clonality Monoclonal
Clone Name RM228
Isotype IgG
Application WB, IHC
Reactivity Human
Package
500 μl ($589)
APPLICATION

Application Note

Immunohistochemistry (IHC): 1:100 dilution, Western Blot (WB): 1:1000 dilution.

Calculated MW

138 kDa. ( Note )

Specificity/Sensitivity

This antibody reacts to Human c-erbB-2/HER-2. This antibody may also react to mouse or rat c-erbB-2/HER-2, as predicted by immunogen homology.
PROPERTIES

Form

Liquid

Buffer

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A peptide corresponding to the C-terminus of Human c-erbB-2/HER-2

Purification

Protein A affinity purified from an animal origin-free culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erb-B2 Receptor Tyrosine Kinase 2 , Cd340 , Her-2 , Her-2/Neu , Her2 , Mln 19 , Neu , Ngl , Tkr1 , Erbb2

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Her2 / ErbB2 antibody [RM228] used in Western Blot (WB). GTX33616

GTX33616 WB Image

Western Blot of MCF-7, PC3, Jurkat, and A375cell lysates using RM228, showed a band of Her2 expressed only in MCF-7 cells.

Anti-Her2 / ErbB2 antibody [RM228] used in Immunohistochemistry (IHC). GTX33616

GTX33616 IHC Image

Immunohistochemical staining of FFPE human breast cancer tissue sections, using Anti-HER2 (c-erbB-2) Rabbit Monoclonal antibody RM228.

REFERENCE
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REVIEW
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Package List Price ($)
$ 589