GeneTex
United States (US)

Her2 / ErbB2 antibody (ready-to-use)

c-ErbB2-antibody-ready-to-use_IHC (Formalin-fixed paraffin-embedded sections)_GTX16932-1_18121409_125.jpg

Cat No. GTX16932

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application IHC-P, AF, IHC-F
Reactivity Human
APPLICATION

Application Note

AF: Use at an assay dependent dilution. IHC-F: Use at an assay dependent dilution. Suggested incubation period of 30 minutes at room temperature. Depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Although not required, heat treatment with 10 mM Citrate buffer, ph 6.0 may enhance the immunostaining in the overfixed tissues. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

138 kDa. ( Note )

Positive Control

Breast carcinoma

Specificity/Sensitivity

This antibody reacts with the c-erbB-2 oncoprotein, a 190 kD protein.
PROPERTIES

Form

Liquid

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Antigen Species

Human

Immunogen

Keyhole limpet hemocyanin conjugated synthetic human peptide c-erbB-2 oncoprotein.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erb-B2 Receptor Tyrosine Kinase 2,Cd340,Her-2,Her-2/Neu,Her2,Mln 19,Neu,Ngl,Tkr1,Erbb2

Cellular Localization

Plasma membraneType I membrane protein

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized.

Database

Research Area

DATA IMAGES
c-ErbB2-antibody-ready-to-use_IHC (Formalin-fixed paraffin-embedded sections)_GTX16932-1_18121409_125.jpg

GTX16932 IHC-P Image

Formalin fixed paraffin embedded human breast carcinoma stained with c-erbB-2 antibody (GTX16932).

Package List Price ($)
$ 319