GeneTex
United States (US)

Her2 / ErbB2 antibody

Comparison Orthogonal
GTX100509_41332_20170817_IHC-P_H_19011506_473.jpg
GTX100509_41332_20160414_WB_19011506_146.jpg
GTX100509_41332_20170818_WB_2_19011506_239.jpg
GTX100509_41332_20170818_WB_19011506_135.jpg

Cat No. GTX100509

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Positive Control

A549 , H1299 , HCT116 , MCF-7 , MDA-MB-231

Specificity/Sensitivity

This antibody detects endogenous Her2/ErbB2 protein. It may cross react slightly with overexpressed EGFR.

Predict Reactivity

Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.07mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Her2 / ErbB2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

CD340 antibody , ERBB2 antibody , HER2 antibody , HER2\/neu antibody , MLN 19 antibody , NEU antibody , NGL antibody , TKR1 antibody , erb-b2 receptor tyrosine kinase 2 antibody

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100509_41332_20170817_IHC-P_H_19011506_473.jpg

GTX100509 IHC-P Image

Comparison

Her2 / ErbB2 antibody [C2C3], C-term detects Her2 / ErbB2 protein at cell membrane in human breast carcinoma by immunohistochemical analysis.
Sample: Strong positive (++), low positive (+) and negative tissue slides cores assessed using Quantitative Digital Pathology.
Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:500, and competitor's antibody (CST#4290) diluted at 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX100509_41332_20160414_WB_19011506_146.jpg

GTX100509 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:10000.

GTX100509_41332_20170818_WB_2_19011506_239.jpg

GTX100509 WB Image

Orthogonal

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100509_41332_20170818_WB_19011506_135.jpg

GTX100509 WB Image

Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169