GeneTex
United States (US)

Her2 / ErbB2 antibody

Cat No. GTX16826

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Human, Rat
APPLICATION

Application Note

For IF: Use at a concentration of 1-5 μg/ml.For IP: Use at a concentration of 1-2 μg/ml. For WB: Use at a concentration of 1-5 μg/ml. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

138 kDa. ( Note )

Positive Control

SK-BR-3 cells
PROPERTIES

Form

Liquid

Buffer

50 mM sodium phosphate buffer, 50% glycerol containing no preservatives

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: LARLLDIDETEYHAD, corresponding to amino acids 866-880 of Human ErbB2 kinase domain.

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erb-B2 Receptor Tyrosine Kinase 2,Cd340,Her-2,Her-2/Neu,Her2,Mln 19,Neu,Ngl,Tkr1,Erbb2

Cellular Localization

Cell Membrane

Background

ErbB2 (also known as Her2 or neu) is a 185 kDa transmembrane receptor tyrosine kinase from the EGFR family that acts to regulate a variety of biological responses including cell growth, differentiation, and tissue development. Ligand binding to the extracellular domain, or overexpression of the receptor leads to phosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Overexpression or abnormal activation of ErbB2 has been found in a variety of tumors including brain, breast, lung and skin cancer. Autophosphorylation of tyrosine 1139 on ErbB2 allows binding of Grb2 and the Src SH2 domain, which allows activation of the Ras, Raf, ERK1&2 and JAK/STAT signaling pathways, respectively.

Database

Research Area

Package List Price ($)
$ 319