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Her2 / ErbB2 (phospho Tyr1139) antibody

Anti-Her2 / ErbB2 (phospho Tyr1139) antibody used in Western Blot (WB). GTX130417
Anti-Her2 / ErbB2 (phospho Tyr1139) antibody used in Western Blot (WB). GTX130417

Cat No. GTX130417

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100μl ($319),
25μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:10000
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Positive Control

A431 ( 200 ng/ml Trichostatin A treatment for 15 min) , A431 (200ng/ml Trichostatin A for 15min)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.094 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human ErbB2 (phospho Tyr1139). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erb-B2 Receptor Tyrosine Kinase 2,Cd340,Her-2,Her-2/Neu,Her2,Mln 19,Neu,Ngl,Tkr1,Erbb2

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Her2 / ErbB2 (phospho Tyr1139) antibody used in Western Blot (WB). GTX130417

GTX130417 WB Image

ErbB2 (phospho Tyr1139) antibody detects ErbB2 (phospho Tyr1139) protein by western blot analysis. Un-treated (-) and treated (+, 200ng/ml Trichostatin A treatment for 15min) A431 whole cell extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ErbB2 (phospho Tyr1139) antibody (GTX130417) at a dilution of 1:10000.

Anti-Her2 / ErbB2 (phospho Tyr1139) antibody used in Western Blot (WB). GTX130417

GTX130417 WB Image

Untreated (–) and treated (+) A431 whole cell extracts (5 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1139) antibody (GTX130417) diluted at 1:10000.

REFERENCE
There are currently no references for Her2 / ErbB2 (phospho Tyr1139) antibody (GTX130417).
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169