APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Neuregulin1 treated MCF-7
Predict Reactivity
Mouse, Rat, Bovine, Cat, Dog, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.88 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr1221/Tyr1222 of human Her2 / ErbB2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886986
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX133445 WB Image
Untreated (–) and treated (+) MCF-7 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody (GTX133445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX133445 IHC-P Image
Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody detects Her2 / ErbB2 (phospho Tyr1221/Tyr1222) protein at cell membrane by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).Sample: Paraffin-embedded human breast carcinoma.Her2 / ErbB2 (phospho Tyr1221/Tyr1222) stained by Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody (GTX133445) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody (GTX133445). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody (GTX133445). Be the first to share your experience with this product.
