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Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody

Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in IHC (Paraffin sections) (IHC-P). GTX133445
Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Western Blot (WB). GTX133445

Cat No. GTX133445

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Positive Control

Neuregulin1 treated MCF-7 whole cell extracts
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human Her2 / ErbB2 (phospho Tyr1221/Tyr1222). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2,CD340,HER-2,HER-2/neu,HER2,MLN 19,NEU,NGL,TKR1

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in IHC (Paraffin sections) (IHC-P). GTX133445

GTX133445 IHC-P Image

Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody detects Her2 / ErbB2 (phospho Tyr1221/Tyr1222) protein at cell membrane by immunohistochemical analysis in the presence of control peptide (left) or antigen-specific peptide (right).
Sample: Paraffin-embedded human breast carcinoma.
Her2 / ErbB2 (phospho Tyr1221/Tyr1222) stained by Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody (GTX133445) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Western Blot (WB). GTX133445

GTX133445 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody (GTX133445) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169