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Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody

Orthogonal
Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50143
Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Western Blot (WB). GTX50143
Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Western Blot (WB). GTX50143

Cat. No. GTX50143

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399)
See all Her2 / ErbB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine1221/1222 (N-L-Y(p)-Y(p)-W) derived from human Her2 / ErbB2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1

Cellular Localization

Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50143

GTX50143 ICC/IF Image

Orthogonal

ICC/IF analysis of methanol-fixed MCF cells using GTX50143 Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody.

Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Western Blot (WB). GTX50143

GTX50143 WB Image

Orthogonal

WB analysis of extracts from MDA cells untreated or treated with EGF using GTX50143 Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody.

Anti-Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody used in Western Blot (WB). GTX50143

GTX50143 WB Image

WB analysis of extracts from HeLa cells treated with EGF or calf intestinal phosphatase (CIP) using GTX50143 Her2 / ErbB2 (phospho Tyr1221/Tyr1222) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399