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Her2 / ErbB2 (phospho Tyr1248) antibody

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Western Blot (WB). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Western Blot (WB). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in ELISA (ELISA). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03358

Cat No. GTX03358

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μl ($379)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:100-1:500
IHC-P 1:50-1:500
ELISA Assay dependent
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from Human ERBB2(Accession P04626), corresponding to amino acid residues P1228-V1255.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1

Cellular Localization

Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of human brain tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Western Blot (WB). GTX03358

GTX03358 WB Image

WB analysis of serum starvation treated MCF7 cell lysate using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody. The lane on the left was treated with blocking peptide.

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of mouse testicular tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of human skin tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of rat appendiceal tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Western Blot (WB). GTX03358

GTX03358 WB Image

WB analysis of various sample lysates using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat spleen tissue lysate treated with blocking peptide.

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of human TB tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of rat ovarian tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in ELISA (ELISA). GTX03358

GTX03358 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

GTX03358 IHC-P Image

IHC-P analysis of mouse gastric tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03358

GTX03358 ICC/IF Image

ICC/IF analysis of PFA-fixed MDA-MB-231 cells using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 379