Her2 / ErbB2 (phospho Tyr1248) antibody

Name: Her2 / ErbB2 (phospho Tyr1248) antibody
Brand: GeneTex
SKU: GTX03358
Price: 399.00 USD

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Western Blot (WB). GTX03358

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX03358

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in ELISA (ELISA). GTX03358

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03358

Cat. No. GTX03358

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, ELISA
Reactivity	Human, Mouse, Rat
Package	100 μl ($399)

See all Her2 / ErbB2 products

Datasheet

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:2000
ICC/IF	1:100-1:500
IHC-P	1:50-1:500
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

138 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from Human ERBB2(Accession P04626), corresponding to amino acid residues P1228-V1255.

Purification

Purified by sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1

Cellular Localization

Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2064 ERBB2
UniProt: P04626 ERBB2

Research Area

DATA IMAGES

GTX03358 WB Image

WB analysis of serum starvation treated MCF7 cell lysate using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody. The lane on the left was treated with blocking peptide.

GTX03358 IHC-P Image

IHC-P analysis of human TB tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 IHC-P Image

IHC-P analysis of human brain tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 IHC-P Image

IHC-P analysis of mouse brain tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 IHC-P Image

IHC-P analysis of rat appendiceal tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 IHC-P Image

IHC-P analysis of mouse testicular tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 IHC-P Image

IHC-P analysis of human skin tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 IHC-P Image

IHC-P analysis of rat ovarian tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 ELISA Image

ELISA analysis of phospho- and non phosho- immunogen peptide using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
P-peptide : phospho-peptide
N-peptide : non-phospho-peptide
Peptides concentration : 1μg/ml

GTX03358 IHC-P Image

IHC-P analysis of rat kidney tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 IHC-P Image

IHC-P analysis of mouse gastric tissue section using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Antigen retireval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

GTX03358 WB Image

WB analysis of various sample lysates using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Lane 1 : Mouse muscle tissue lysate
Lane 2 : Rat spleen tissue lysate
Lane 3 : Rat spleen tissue lysate treated with blocking peptide.

GTX03358 ICC/IF Image

ICC/IF analysis of PFA-fixed MDA-MB-231 cells using GTX03358 Her2 / ErbB2 (phospho Tyr1248) antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

REFERENCE

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REVIEW

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Package	List Price ($)
100 μl	$ 399
Qty

Her2 / ErbB2 (phospho Tyr1248) antibody

Cat. No. GTX03358

Host

Clonality

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX03358 WB Image

GTX03358 IHC-P Image

GTX03358 IHC-P Image

GTX03358 IHC-P Image

GTX03358 IHC-P Image

GTX03358 IHC-P Image

GTX03358 IHC-P Image

GTX03358 IHC-P Image

GTX03358 ELISA Image

GTX03358 IHC-P Image

GTX03358 IHC-P Image

GTX03358 WB Image

GTX03358 ICC/IF Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.