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Her2 / ErbB2 (phospho Tyr1248) antibody

Orthogonal
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX50146
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX50146
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50146
Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Western Blot (WB). GTX50146

Cat. No. GTX50146

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all Her2 / ErbB2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of tyrosine1248 (P-E-Y(p)-L-G) derived from human Her2 / ErbB2.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 2 , CD340 , HER-2 , HER-2/neu , HER2 , MLN 19 , NEU , NGL , TKR1

Cellular Localization

Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX50146

GTX50146 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50146 Her2 / ErbB2 (phospho Tyr1248) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in IHC (Paraffin sections) (IHC-P). GTX50146

GTX50146 IHC-P Image

Orthogonal

IHC-P analysis of human lung carcinoma tissue using GTX50146 Her2 / ErbB2 (phospho Tyr1248) antibody.

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50146

GTX50146 ICC/IF Image

ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50146 Her2 / ErbB2 (phospho Tyr1248) antibody.

Anti-Her2 / ErbB2 (phospho Tyr1248) antibody used in Western Blot (WB). GTX50146

GTX50146 WB Image

Orthogonal

WB analysis of extracts from HepG2 cells treated with PMA and MCF cells treated with EGF using GTX50146 Her2 / ErbB2 (phospho Tyr1248) antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399