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Her3 / ErbB3 antibody [2F9]

Anti-Her3 / ErbB3 antibody [2F9] used in Western Blot (WB). GTX83364
Anti-Her3 / ErbB3 antibody [2F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83364

Cat. No. GTX83364

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F9

Isotype

 IgG1

Application

 WB, ICC/IF, IP, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Her3 / ErbB3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IP Assay dependent
ELISA 1/10000
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant extracellular fragment of human ERBB3 (aa22-369) fused with hIgGFc tag expressed in HEK293 cells.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11167293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 3 , ErbB-3 , FERLK , HER3 , LCCS2 , MDA-BF-1 , c-erbB-3 , c-erbB3 , erbB3-S , p180-ErbB3 , p45-sErbB3 , p85-sErbB3

Background

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her3 / ErbB3 antibody [2F9] used in Western Blot (WB). GTX83364

GTX83364 WB Image

WB analysis of human ERBB3 (aa22-369)-hIgGFc trasfected HEK293 cell lysate using GTX83364 Her3 / ErbB3 antibody [2F9].

Anti-Her3 / ErbB3 antibody [2F9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83364

GTX83364 ICC/IF Image

ICC/IF analysis of HEK293 cells trasfected with extracellular ERBB3 (aa22-369)-hIgGFc using GTX83364 Her3 / ErbB3 antibody [2F9].
Green : Her3 / ErbB3
Blue: DRAQ5 fluorescent DNA dye

REFERENCE

Application Reference

Tsai CH et al. Cancer Res 2016; 76 (19) : 5756-5767 Metastatic Progression of Prostate Cancer Is Mediated by Autonomous Binding of Galectin-4-O-Glycan to Cancer Cells.
Application : WB, IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399