GeneTex
United States (US)

Her3 / ErbB3 antibody [RTJ1]

Cat No. GTX28758

Host Mouse
Clonality Monoclonal
Clone Name RTJ1
Isotype IgM
Application WB, IHC-P, IHC-Fr
Reactivity Human
APPLICATION

Application Note

This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. In a standard three-step streptavidin-biotin method a dilution of 1:40 may be used as a guideline. When using paraffin-embedded and formalin-fixed tissues, high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. This product can also be used on frozen tissue sections. The optimal conditions should be determined by the individual laboratory.

Calculated MW

148 kDa. ( Note )

Specificity/Sensitivity

This antibody is specific against c-erbB-3 oncoprotein that is expressed in a variety of human normal, fetal and neoplastic epithelial tissues.
PROPERTIES

Form

Liquid

Buffer

10mM PBS, pH 7.4, with 0.2% BSA and <0.1% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to a site on the cytoplasmic domain of the human c-erbB-3 oncoprotein.

Purification

Purified
From hybridoma culture supernatant

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

c-erbB-3, c-erbB3, p45-sErbB3, p85-sErbB3, ErbB-3, MDABF1, ErbB3, P21860, erbB3-S, p180ErbB3, p85sErbB3, erbB3S, cerbB3, p45sErbB3, 190151, MGC88033, 2065, HER3, MDA-BF-1, p180-ErbB3, ErbB 3, c erbB3, erbB3 S, c erbB 3, MDA BF 1, p45 sErbB3, p85 sErbB3, p

Cellular Localization

Type I membrane protein and Secreted

Background

This antibody reacts with normal gastrointestinal epithelium as well as gastrointestinal and breast tumors, resulting in a cytoplasmic staining pattern.

Database

Research Area