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Her3 / ErbB3 (phospho Tyr1328) antibody

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32388
Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Western Blot (WB). GTX32388
Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in IHC (Paraffin sections) (IHC-P). GTX32388

Cat No. GTX32388

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Her3 / ErbB3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 3 , ErbB-3 , FERLK , HER3 , LCCS2 , MDA-BF-1 , c-erbB-3 , c-erbB3 , erbB3-S , p180-ErbB3 , p45-sErbB3 , p85-sErbB3

Background

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32388

GTX32388 ICC/IF Image

ICC/IF analysis of formalin-fixed MCF7 cells using GTX32388 Her3 / ErbB3 (phospho Tyr1328) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Western Blot (WB). GTX32388

GTX32388 WB Image

WB analysis of EGF-treated A431 (A), Raw264.7 (B) whole cell lysates using GTX32388 Her3 / ErbB3 (phospho Tyr1328) antibody.

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in IHC (Paraffin sections) (IHC-P). GTX32388

GTX32388 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32388 Her3 / ErbB3 (phospho Tyr1328) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

There are currently no references for Her3 / ErbB3 (phospho Tyr1328) antibody (GTX32388). Be the first to share your publications with this product.

REVIEW

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Package List Price ($)
$ 319