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Her3 / ErbB3 (phospho Tyr1328) antibody

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Western Blot (WB). GTX38571
Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in IHC (Paraffin sections) (IHC-P). GTX38571
Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38571

Cat. No. GTX38571

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
50 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:200
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

148 kDa. ( Note )

Product Note

This antibody detects endogenous levels of Her3/ErbB3 only when phosphorylated at tyrosine 1328.

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human Her3/ErbB3 around the phosphorylation site of tyrosine 1328 (P -D-Y(p)-W-H).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 3 , ErbB-3 , FERLK , HER3 , LCCS2 , MDA-BF-1 , c-erbB-3 , c-erbB3 , erbB3-S , p180-ErbB3 , p45-sErbB3 , p85-sErbB3

Background

This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Western Blot (WB). GTX38571

GTX38571 WB Image

WB analysis of A431 cell lysate using GTX38571 Her3 / ErbB3 (phospho Tyr1328) antibody.

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in IHC (Paraffin sections) (IHC-P). GTX38571

GTX38571 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX38571 Her3 / ErbB3 (phospho Tyr1328) antibody.

Anti-Her3 / ErbB3 (phospho Tyr1328) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX38571

GTX38571 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX38571 Her3 / ErbB3 (phospho Tyr1328) antibody.

REFERENCE

There are currently no references for Her3 / ErbB3 (phospho Tyr1328) antibody (GTX38571). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Her3 / ErbB3 (phospho Tyr1328) antibody (GTX38571). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399