APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:1000 |
| 1:100-1:200 |
| 1:50-1:100 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Mouse, Rat(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide sequence around phosphorylation site of tyrosine 1328 (P-D-Y(p)-W-H) derived from human Her3 / ErbB3.
Purification
Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
erb-b2 receptor tyrosine kinase 3 , ErbB-3 , FERLK , HER3 , LCCS2 , MDA-BF-1 , c-erbB-3 , c-erbB3 , erbB3-S , p180-ErbB3 , p45-sErbB3 , p85-sErbB3
Background
This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. It therefore can bind this ligand but not convey the signal into the cell through protein phosphorylation. However, it does form heterodimers with other EGF receptor family members which do have kinase activity. Heterodimerization leads to the activation of pathways which lead to cell proliferation or differentiation. Amplification of this gene and/or overexpression of its protein have been reported in numerous cancers, including prostate, bladder, and breast tumors. Alternate transcriptional splice variants encoding different isoforms have been characterized. One isoform lacks the intermembrane region and is secreted outside the cell. This form acts to modulate the activity of the membrane-bound form. Additional splice variants have also been reported, but they have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX50341 WB Image
WB analysis of extracts from A431 cells untreated or treated with EGF using GTX50341 Her3 / ErbB3 (phospho Tyr1328) antibody.
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GTX50341 IHC-P Image
IHC-P analysis of human breast carcinoma tissue using GTX50341 Her3 / ErbB3 (phospho Tyr1328) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide
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GTX50341 ICC/IF Image
ICC/IF analysis of methanol-fixed MCF-7 cells using GTX50341 Her3 / ErbB3 (phospho Tyr1328) antibody.
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REFERENCE
There are currently no references for Her3 / ErbB3 (phospho Tyr1328) antibody (GTX50341). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Her3 / ErbB3 (phospho Tyr1328) antibody (GTX50341). Be the first to share your experience with this product.
