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Her4 / ErbB4 antibody [C1C3]

Anti-Her4 / ErbB4 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Immunoprecipitation (IP). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

Cat No. GTX111276

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

A431 , mouse brain , rat brain

Predict Reactivity

Chicken, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Her4 / ErbB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950216

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 4 , ALS19 , HER4 , p180erbB4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Her4 / ErbB4 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111276

GTX111276 ICC/IF Image

ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (GTX111276) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Her4 / ErbB4 antibody [C1C3] used in Immunoprecipitation (IP). GTX111276

GTX111276 IP Image

Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 μg of Her4 / ErbB4 antibody [C1C3] (GTX111276).
Western blot analysis was performed using Her4 / ErbB4 antibody [C1C3] (GTX111276) and diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

GTX111276 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

GTX111276 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Her4 / ErbB4 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111276

GTX111276 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4(v-erb-A)(GTX111276) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

GTX111276 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf