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Her4 / ErbB4 antibody [C1C3]

Overexpression
Anti-Her4 / ErbB4 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Immunoprecipitation (IP). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276
Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

Cat. No. GTX111276

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Her4 / ErbB4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

Positive Control

mouse brain , rat brain , DDDDK-tagged ERBB4-transfected 293T

Predict Reactivity

Chicken(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.97 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the Intracellular domain of human Her4 / ErbB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950216

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 4 , ALS19 , HER4 , p180erbB4

Cellular Localization

Membrane; Single-pass type I membrane protein

Background

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Her4 / ErbB4 antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111276

GTX111276 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4(v-erb-A)(GTX111276) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Her4 / ErbB4 antibody [C1C3] used in Immunoprecipitation (IP). GTX111276

GTX111276 IP Image

Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 μg of Her4 / ErbB4 antibody [C1C3] (GTX111276).
Western blot analysis was performed using Her4 / ErbB4 antibody [C1C3] (GTX111276) and diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Anti-Her4 / ErbB4 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111276

GTX111276 ICC/IF Image

ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (GTX111276) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

GTX111276 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

GTX111276 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Her4 / ErbB4 antibody [C1C3] used in Western Blot (WB). GTX111276

GTX111276 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Hsu FN et al. Am J Physiol Endocrinol Metab 2011; 300 (5) : E902-8 The significance of Her2 on androgen receptor protein stability in the transition of androgen requirement in prostate cancer cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169