Her4 / ErbB4 antibody [HFR1]

Name: Her4 / ErbB4 antibody [HFR1]
Brand: GeneTex
SKU: GTX80811
Price: 399.00 USD

Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811

Anti-Her4 / ErbB4 antibody [HFR1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80811

Anti-Her4 / ErbB4 antibody [HFR1] used in Flow cytometry (FACS). GTX80811

Anti-Her4 / ErbB4 antibody [HFR1] used in Western Blot (WB). GTX80811

Cat. No. GTX80811

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	HFR1
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, IHC
Reactivity	Human, Mouse
Package	200 μg ($399)

See all Her4 / ErbB4 products

View product citations for antibody GTX80811 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:100-1:500
ICC/IF	1:50
IHC-P	1:100
IHC-Fr	Assay dependent
FACS	0.5-2 μg/test
IP	Assay dependent
IHC	Assay dependent

Not tested in other applications.

Calculated MW

147 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide: C R(1250) S T L Q H P D Y L Q E Y S T(1264)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 4 , ALS19 , HER4 , p180erbB4

Cellular Localization

Cell membrane,Mitochondrion

Background

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2066 ERBB4
UniProt: Q15303 ERBB4

Research Area

DATA IMAGES

GTX80811 IHC-P Image

IHC-P analysis of mouse placenta tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX80811 IHC-P Image

IHC-P analysis of human heart tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX80811 ICC/IF Image

ICC/IF analysis of A431 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX80811 ICC/IF Image

ICC/IF analysis of SK-BR-3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

GTX80811 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX80811 FACS Image

FACS analysis of MCF-7 cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 1ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

GTX80811 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX80811 FACS Image

FACS analysis of Hela cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 1ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

GTX80811 WB Image

WB analysis of 25 ug of MDA-MB-453 (Lane 1) and T47D (Lane 2) cell lysates using GTX80811 Her4 / ErbB4 antibody [HFR1].
Dilution : 1:300

GTX80811 FACS Image

FACS analysis of NIH-3T3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 0.5ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

GTX80811 IHC-P Image

IHC-P analysis of human cervix carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX80811 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

GTX80811 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

REFERENCE

Application Reference

Spahic H et al. Int J Mol Sci 2022; 24 (1) Dysregulation of ErbB4 Signaling Pathway in the Dorsal Hippocampus after Neonatal Hypoxia-Ischemia and Late Deficits in PV(+) Interneurons, Synaptic Plasticity and Working Memory.

Application : IHC-Fr

Reactivity : Mouse

Submit a Reference

REVIEW

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Application Reference

Application : IHC-Fr

Reactivity : Mouse

SDS
PBS.pdf
Sodium Azide.pdf

Package	List Price ($)
200 μg	$ 399
Qty

Her4 / ErbB4 antibody [HFR1]

Cat. No. GTX80811

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX80811 IHC-P Image

GTX80811 IHC-P Image

GTX80811 ICC/IF Image

GTX80811 ICC/IF Image

GTX80811 IHC-P Image

GTX80811 FACS Image

GTX80811 IHC-P Image

GTX80811 FACS Image

GTX80811 WB Image

GTX80811 FACS Image

GTX80811 IHC-P Image

GTX80811 IHC-P Image

GTX80811 ICC/IF Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.