ICC/IF analysis of NIH-3T3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

IHC-P analysis of human breast carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

IHC-P analysis of human cervix carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

FACS analysis of NIH-3T3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 0.5ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

WB analysis of 25 ug of MDA-MB-453 (Lane 1) and T47D (Lane 2) cell lysates using GTX80811 Her4 / ErbB4 antibody [HFR1].
Dilution : 1:300

FACS analysis of Hela cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 1ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

IHC-P analysis of human breast carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

FACS analysis of MCF-7 cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 1ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

IHC-P analysis of human pancreas tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

ICC/IF analysis of SK-BR-3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

ICC/IF analysis of A431 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

IHC-P analysis of human heart tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

IHC-P analysis of mouse placenta tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100