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Her4 / ErbB4 antibody [HFR1]

Anti-Her4 / ErbB4 antibody [HFR1] used in Flow cytometry (FACS). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in Flow cytometry (FACS). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in Western Blot (WB). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80811
Anti-Her4 / ErbB4 antibody [HFR1] used in Flow cytometry (FACS). GTX80811

Cat No. GTX80811

Host

Mouse

Clonality

Monoclonal

Clone Name

HFR1

Isotype

IgG2b

Application

WB, ICC/IF, IHC-P, FACS, IP, IHC

Reactivity

Human, Mouse
Package
200 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:500
ICC/IF 1:50
IHC-P 1:100
FACS 0.5-2 μg/test
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Calculated MW

147 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic Peptide: C R(1250) S T L Q H P D Y L Q E Y S T(1264)

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

erb-b2 receptor tyrosine kinase 4 , ALS19 , HER4 , p180erbB4

Cellular Localization

Cell membrane,Mitochondrion

Background

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her4 / ErbB4 antibody [HFR1] used in Flow cytometry (FACS). GTX80811

GTX80811 FACS Image

FACS analysis of MCF-7 cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 1ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

Anti-Her4 / ErbB4 antibody [HFR1] used in Flow cytometry (FACS). GTX80811

GTX80811 FACS Image

FACS analysis of Hela cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 1ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811

GTX80811 IHC-P Image

IHC-P analysis of mouse placenta tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811

GTX80811 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811

GTX80811 IHC-P Image

IHC-P analysis of human pancreas tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811

GTX80811 IHC-P Image

IHC-P analysis of human cervix carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Her4 / ErbB4 antibody [HFR1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80811

GTX80811 ICC/IF Image

ICC/IF analysis of A431 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-Her4 / ErbB4 antibody [HFR1] used in Western Blot (WB). GTX80811

GTX80811 WB Image

WB analysis of 25 ug of MDA-MB-453 (Lane 1) and T47D (Lane 2) cell lysates using GTX80811 Her4 / ErbB4 antibody [HFR1].
Dilution : 1:300

Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811

GTX80811 IHC-P Image

IHC-P analysis of human heart tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-Her4 / ErbB4 antibody [HFR1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80811

GTX80811 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-Her4 / ErbB4 antibody [HFR1] used in IHC (Paraffin sections) (IHC-P). GTX80811

GTX80811 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX80811 Her4 / ErbB4 antibody [HFR1].
Right : Primary antibody
Left : Negative control without primary antibody
Antigen retrieval : 10mM sodium citrate (pH 6.0), microwaved for 8-15 min
Dilution : 1:100

Anti-Her4 / ErbB4 antibody [HFR1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80811

GTX80811 ICC/IF Image

ICC/IF analysis of SK-BR-3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1]. Cells were probed without (left) or with(right) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute
Dilution : 1:50 and incubated overnight

Anti-Her4 / ErbB4 antibody [HFR1] used in Flow cytometry (FACS). GTX80811

GTX80811 FACS Image

FACS analysis of NIH-3T3 cells using GTX80811 Her4 / ErbB4 antibody [HFR1] compared to an isotype control (blue).
Dilution : 0.5ug/test for 40 min at room temperature
Fixation : 2% paraformaldehyde

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf