GeneTex
United States (US)

Her4 / ErbB4 antibody

GTX15137_20170930_IHC-P_18121410_658.jpg
GTX15137_20170930_WB_18121410_629.jpg

Cat No. GTX15137

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

IHC-P: Use at a dilution of 1/50 for 10 min at RT. Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB: Use at an assay dependent dilution. Predicted molecular weight: 146.8 kDa.
Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

147 kDa. ( Note )

Positive Control

Breast carcinoma or skeletal muscle
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erb-B2 Receptor Tyrosine Kinase 4,Als19,Her4,P180Erbb4,Erbb4

Cellular Localization

Type I membrane protein

Background

ErbB 4 is the fourth member of class I receptor kinase family. EbbB 4 is most predominantly expressed in several breast carcinoma cell lines, and in normal skeletal muscle, heart, pituitary, brain, and cerebellum. Breast tumor cell lines T47-D, MDA-MB-453, BT-474 and H3396 are found to have the highest levels of mRNA, and intermediate levels are seen in MCF-7, MDAMB-330 and MDA-MB-361. Expression of ErbB 4 is low or absent in some breast tumor cell lines such as MDA-MB-231, MDA-MB-157, MDA-MB-468, and SKBR-3.

Database

Research Area

DATA IMAGES
GTX15137_20170930_IHC-P_18121410_658.jpg

GTX15137 IHC-P Image

IHC-P analysis of human breast carcinoma using GTX15137 Her4 / ErbB4 antibody.

GTX15137_20170930_WB_18121410_629.jpg

GTX15137 WB Image

WB analysis of A431 cell lysate using GTX15137 Her4 / ErbB4 antibody.

Package List Price ($)
$ 319