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Her4 / ErbB4 (phospho Tyr1284) antibody

Anti-Her4 / ErbB4 (phospho Tyr1284) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32389
Anti-Her4 / ErbB4 (phospho Tyr1284) antibody used in IHC (Paraffin sections) (IHC-P). GTX32389
Anti-Her4 / ErbB4 (phospho Tyr1284) antibody used in Western Blot (WB). GTX32389

Cat No. GTX32389

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

147 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Her4 / ErbB4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Erb-B2 Receptor Tyrosine Kinase 4,Als19,Her4,P180Erbb4,Erbb4

Cellular Localization

Cell membrane,Mitochondrion,Nucleus

Background

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Her4 / ErbB4 (phospho Tyr1284) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32389

GTX32389 ICC/IF Image

ICC/IF analysis of formalin-fixed MCF7 cells using GTX32389 Her4 / ErbB4 (phospho Tyr1284) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Her4 / ErbB4 (phospho Tyr1284) antibody used in IHC (Paraffin sections) (IHC-P). GTX32389

GTX32389 IHC-P Image

IHC-P analysis of formalin fixed human breast cancer tissue section using GTX32389 Her4 / ErbB4 (phospho Tyr1284) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Her4 / ErbB4 (phospho Tyr1284) antibody used in Western Blot (WB). GTX32389

GTX32389 WB Image

WB analysis of MCF7 (A), EGF-treated A431 (B) whole cell lysates using GTX32389 Her4 / ErbB4 (phospho Tyr1284) antibody.

Package List Price ($)
$ 319