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Hex antibody [3C4]

Overexpression
Anti-Hex antibody [3C4] used in Western Blot (WB). GTX84372
Anti-Hex antibody [3C4] used in Western Blot (WB). GTX84372
Anti-Hex antibody [3C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84372
Anti-Hex antibody [3C4] used in Western Blot (WB). GTX84372
Anti-Hex antibody [3C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84372

Cat. No. GTX84372

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3C4

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($519)
See all Hex products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:50-1:100
ICC/IF 1:100
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 100-270 of human Hex

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hematopoietically expressed homeobox , HEX , HMPH , HOX11L-PEN , PRH , PRHX

Cellular Localization

Nucleus

Background

Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels | acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL.

Database

Research Area

DATA IMAGES

Anti-Hex antibody [3C4] used in Western Blot (WB). GTX84372

GTX84372 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Hex plasmid (Right) or empty vector (Left) for 48 hrs using GTX84372 Hex antibody [3C4].
Loading : 5 ug per lane

Anti-Hex antibody [3C4] used in Western Blot (WB). GTX84372

GTX84372 WB Image

Overexpression

WB analysis of HEK-293T cells transfected with human Hex using GTX84372 Hex antibody [3C4].
Dilution : 1:100

Anti-Hex antibody [3C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84372

GTX84372 ICC/IF Image

ICC/IF analysis of HeLa cells transiently transfected with Hex plasmid using GTX84372 Hex antibody [3C4].

Anti-Hex antibody [3C4] used in Western Blot (WB). GTX84372

GTX84372 WB Image

WB analysis of various cell lines using GTX84372 Hex antibody [3C4].
Loading : 35 ug per lane
Dilution : 1:50

Anti-Hex antibody [3C4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84372

GTX84372 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX84372 Hex antibody [3C4].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519