Hexanoyl-Lysine adduct antibody [5D9]

Cat. No. GTX02872

Host	Mouse
Clonality	Monoclonal
Clone Name	5D9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Species independent
Package	100 μg ($489)

See all Hexanoyl-Lysine products

Datasheet

View product citations for antibody GTX02872 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IHC-P	Assay dependent
FACS	Assay dependent
ELISA	Assay dependent

Not tested in other applications.

Product Note

Specific for Hexanoyl-Lysine adduct (HEL) modified peptides and proteins. Does not detect free Hexanoyl-Lysine. Does not cross-react with Acrolein, Crotonaldehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy none.

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Synthetic Hexanoyl modified Keyhole Limpet Hemocyanin (KLH).

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX02872 WB Image

WB analysis of HeLa cell lysate using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Lane 1 : Molecular Weight Ladder (MW)
Lane 2 : HeLa cell lysate
Lane 3 : H₂O₂ treated HeLa cell lysate
Loading : 12 μg
Dilution : 1:1000

GTX02872 WB Image

WB analysis of various samples using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Lane 1 : Molecular Weight Ladder (MW)
Lane 2 : Hexanoyl Lysine-BSA
Lane 3 : BSA
Loading : 0.5 μg
Dilution : 1:1000

GTX02872 IHC-P Image

IHC-P analysis of rat heart tissue using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Dilution : 1:25
Green : Primary antibody
Blue : DAPI
Red : Actin

GTX02872 FACS Image

FACS analysis of 90% Methanol-fixed SH-SY5Y cells using GTX02872 Hexanoyl-Lysine adduct antibody [5D9]. Cells were subject to oxidative stress by treating with 250 μM H₂O₂ for 24 hours.
Dilution : 1:50

GTX02872 ICC/IF Image

ICC/IF analysis of 5% Formaldehyde-fixed HEK293 cell using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Panel A,C,E,G : Untreated cells
Panel B,D,F,H : Cells cultured overnight with 50 μM H₂O₂
Green : Primary antibody
Red : F-actin
Blue : DAPI
Dilution : 1:50

GTX02872 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX02872 Hexanoyl-Lysine adduct antibody [5D9].
Dilution : 1:100
Green : Primary antibody
Blue : DAPI

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 489

Qty

Hexanoyl-Lysine adduct antibody [5D9]

Cat. No. GTX02872

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Immunogen

Purification

Conjugation

Note

TARGET

Research Area

DATA IMAGES

GTX02872 WB Image

GTX02872 WB Image

GTX02872 IHC-P Image

GTX02872 FACS Image

GTX02872 ICC/IF Image

GTX02872 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.