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Hexokinase 1 antibody [3A10]

Orthogonal
Anti-Hexokinase 1 antibody [3A10] used in Flow cytometry (FACS). GTX82790
Anti-Hexokinase 1 antibody [3A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82790
Anti-Hexokinase 1 antibody [3A10] used in IHC (Paraffin sections) (IHC-P). GTX82790
Anti-Hexokinase 1 antibody [3A10] used in Western Blot (WB). GTX82790
Anti-Hexokinase 1 antibody [3A10] used in Western Blot (WB). GTX82790

Cat. No. GTX82790

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A10

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Hexokinase 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human HK1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11163113

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase 1 , HK , HK1-ta , HK1-tb , HK1-tc , HKD , HKI , HMSNR , HXK1 , RP79 , hexokinase

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-Hexokinase 1 antibody [3A10] used in Flow cytometry (FACS). GTX82790

GTX82790 FACS Image

FACS analysis of K562 cells using GTX82790 Hexokinase 1 antibody [3A10].
Green : Hexokinase 1
Purple : negative control

Anti-Hexokinase 1 antibody [3A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82790

GTX82790 ICC/IF Image

ICC/IF analysis of NIH3T3 cells using GTX82790 Hexokinase 1 antibody [3A10].
Green : Hexokinase 1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-Hexokinase 1 antibody [3A10] used in IHC (Paraffin sections) (IHC-P). GTX82790

GTX82790 IHC-P Image

IHC-P analysis of human kidney tissue using GTX82790 Hexokinase 1 antibody [3A10].

Anti-Hexokinase 1 antibody [3A10] used in Western Blot (WB). GTX82790

GTX82790 WB Image

WB analysis of Jurkat (1), HeLa (2), HepG2 (3) and NIH3T3 (4) cell lysate using GTX82790 Hexokinase 1 antibody [3A10].

Anti-Hexokinase 1 antibody [3A10] used in Western Blot (WB). GTX82790

GTX82790 WB Image

Orthogonal

The data was published in the journal Viruses in 2018. PMID: 29518929

REFERENCE

Application Reference

Kavanagh Williamson M et al. Viruses 2018; 10 (3) Upregulation of Glucose Uptake and Hexokinase Activity of Primary Human CD4+ T Cells in Response to Infection with HIV-1.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399