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Hexokinase 1 antibody [4D7]

Anti-Hexokinase 1 antibody [4D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53727
Anti-Hexokinase 1 antibody [4D7] used in Western Blot (WB). GTX53727

Cat No. GTX53727

Host

Mouse

Clonality

Monoclonal

Clone Name

4D7

Isotype

IgG2a

Application

WB, ICC/IF, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein. The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence assay to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1,000.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Hexokinase 1 (1-917aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Hexokinase 1 , Hk , Hk1-Ta , Hk1-Tb , Hk1-Tc , Hkd , Hki , Hmsnr , Hxk1 , Rp79 , Hexokinase , Hk1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-Hexokinase 1 antibody [4D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53727

GTX53727 ICC/IF Image

ICC/IF analysis of HeLa cells using Hexokinase antibody at a dilution of 1:1,000 (green) and Hoechst 33342 (blue).

Anti-Hexokinase 1 antibody [4D7] used in Western Blot (WB). GTX53727

GTX53727 WB Image

WB analysis of (A) recombinant protein (20ng or 100ng) of Hexokinase four isoform and (B) the indicated lysates (20ug) using Hexokinase antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319