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Hexokinase 1 antibody [4D7]

Anti-Hexokinase 1 antibody [4D7] used in Western Blot (WB). GTX53727
Anti-Hexokinase 1 antibody [4D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53727

Cat. No. GTX53727

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4D7

Isotype

 IgG2a

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all Hexokinase 1 products

APPLICATION

Application Note

The antibody recognizes all four isoforms of Hexokinase (1~4) in recombinant protein. The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence assay to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution is 1:1,000.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Hexokinase 1 (1-917aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase 1 , HK , HK1-ta , HK1-tb , HK1-tc , HKD , HKI , HMSNR , HXK1 , RP79 , hexokinase

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-Hexokinase 1 antibody [4D7] used in Western Blot (WB). GTX53727

GTX53727 WB Image

WB analysis of (A) recombinant protein (20ng or 100ng) of Hexokinase four isoform and (B) the indicated lysates (20ug) using Hexokinase antibody at a dilution of 1:1,000.

Anti-Hexokinase 1 antibody [4D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX53727

GTX53727 ICC/IF Image

ICC/IF analysis of HeLa cells using Hexokinase antibody at a dilution of 1:1,000 (green) and Hoechst 33342 (blue).

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399