APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human HK1 expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hexokinase 1 , HK , HK1-ta , HK1-tb , HK1-tc , HKD , HKI , HMSNR , HXK1 , RP79 , hexokinase
Cellular Localization
Mitochondrion outer membrane
Background
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]
Database
Research Area
DATA IMAGES
![Anti-Hexokinase 1 antibody [7A7] used in Western Blot (WB). GTX82795](/templates/default/js/lightbox/images/loading.gif "Anti-Hexokinase 1 antibody [7A7] used in Western Blot (WB). GTX82795")
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GTX82795 WB Image
WB analysis of Jurkat (1), HeLa (2), HepG2 (3), MCF-7 (4) and PC-12 (5) cell lysate using GTX82795 Hexokinase 1 antibody [7A7].
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GTX82795 IHC-P Image
IHC-P analysis of human salivary gland tissue (left) and kidney tissue (right) using GTX82795 Hexokinase 1 antibody [7A7].
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GTX82795 ICC/IF Image
ICC/IF analysis of NIH3T3 cells using GTX82795 Hexokinase 1 antibody [7A7].
Green : Hexokinase 1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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REFERENCE
There are currently no references for Hexokinase 1 antibody [7A7] (GTX82795). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Hexokinase 1 antibody [7A7] (GTX82795). Be the first to share your experience with this product.
![Anti-Hexokinase 1 antibody [7A7] used in Western Blot (WB). GTX82795](/upload/website/prouct_img/small/GTX82795/GTX82795_20170912_WB_w_23061322_129.webp "Anti-Hexokinase 1 antibody [7A7] used in Western Blot (WB). GTX82795")
![Anti-Hexokinase 1 antibody [7A7] used in IHC (Paraffin sections) (IHC-P). GTX82795](/upload/website/prouct_img/small/GTX82795/GTX82795_20170912_IHC-P_w_23061322_691.webp "Anti-Hexokinase 1 antibody [7A7] used in IHC (Paraffin sections) (IHC-P). GTX82795")
![Anti-Hexokinase 1 antibody [7A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82795](/upload/website/prouct_img/small/GTX82795/GTX82795_20170912_ICCIF_w_23061322_538.webp "Anti-Hexokinase 1 antibody [7A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82795")
![Anti-Hexokinase 1 antibody [7A7] used in Western Blot (WB). GTX82795](/upload/website/prouct_img/normal/GTX82795/GTX82795_20170912_WB_w_23061322_129.webp "Anti-Hexokinase 1 antibody [7A7] used in Western Blot (WB). GTX82795")
![Anti-Hexokinase 1 antibody [7A7] used in IHC (Paraffin sections) (IHC-P). GTX82795](/upload/website/prouct_img/normal/GTX82795/GTX82795_20170912_IHC-P_w_23061322_691.webp "Anti-Hexokinase 1 antibody [7A7] used in IHC (Paraffin sections) (IHC-P). GTX82795")
![Anti-Hexokinase 1 antibody [7A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82795](/upload/website/prouct_img/normal/GTX82795/GTX82795_20170912_ICCIF_w_23061322_538.webp "Anti-Hexokinase 1 antibody [7A7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82795")