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Hexokinase 1 antibody

Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31625
Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31625
Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX31625

Cat No. GTX31625

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

Rat Bladder Tissue Lysate(GTX24031)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Hexokinase 1 antibody was raised against a 16 amino acid peptide near the carboxy terminus of human Hexokinase 1.The immunogen is located within amino acids 830 - 880 of Hexokinase 1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hexokinase 1 , HK , HK1-ta , HK1-tb , HK1-tc , HKD , HKI , HMSNR , HXK1 , RP79 , hexokinase

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31625

GTX31625 IHC-P Image

IHC-P analysis of rat stomach tissue using GTX31625 Hexokinase 1 antibody.
Working concentration : 5 μg/ml

Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31625

GTX31625 IHC-P Image

IHC-P analysis of rat stomach tissue using GTX31625 Hexokinase 1 antibody.
Working concentration : 20 μg/ml

Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX31625

GTX31625 WB Image

WB analysis of rat bladder tissue lysate using GTX31625 Hexokinase 1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339