APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human HK1 (NP_000179.2).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
hexokinase 1 , HK , HK1-ta , HK1-tb , HK1-tc , HKD , HKI , HMSNR , HXK1 , RP79 , hexokinase
Cellular Localization
Mitochondrion outer membrane
Background
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]
Database
Research Area
DATA IMAGES
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GTX54080 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX54080 Hexokinase 1 antibody.
Blue : DAPI
Dilution : 1:100
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GTX54080 IHC-P Image
IHC-P analysis of rat kidney tissue using GTX54080 Hexokinase 1 antibody.
Dilution : 1:150
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GTX54080 WB Image
WB analysis of various sample lysates using GTX54080 Hexokinase 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54080 WB Image
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX54080 Hexokinase 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX54080 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX54080 Hexokinase 1 antibody.
Dilution : 1:150
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REFERENCE
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REVIEW
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