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Hexokinase 1 antibody

Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX54080
Anti-Hexokinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54080
Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX54080
Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54080
Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54080

Cat. No. GTX54080

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human HK1 (NP_000179.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

hexokinase 1 , HK , HK1-ta , HK1-tb , HK1-tc , HKD , HKI , HMSNR , HXK1 , RP79 , hexokinase

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX54080

GTX54080 WB Image

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX54080 Hexokinase 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Hexokinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54080

GTX54080 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX54080 Hexokinase 1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX54080

GTX54080 WB Image

WB analysis of various sample lysates using GTX54080 Hexokinase 1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54080

GTX54080 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX54080 Hexokinase 1 antibody.
Dilution : 1:150

Anti-Hexokinase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54080

GTX54080 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX54080 Hexokinase 1 antibody.
Dilution : 1:150

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399