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Hexokinase 1 antibody

Anti-Hexokinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54080
Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX54080

Cat No. GTX54080

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
Package
100 μg ($319)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:200
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human HK1 (NP_000179.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hexokinase 1,Hk,Hk1-Ta,Hk1-Tb,Hk1-Tc,Hkd,Hki,Hmsnr,Hxk1,Rp79,Hexokinase,Hk1

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES
Anti-Hexokinase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54080

GTX54080 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX54080 Hexokinase 1 antibody.
Blue : DAPI

Anti-Hexokinase 1 antibody used in Western Blot (WB). GTX54080

GTX54080 WB Image

WB analysis of various samples using GTX54080 Hexokinase 1 antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE
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REVIEW
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Package List Price ($)
$ 319