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Hexokinase II antibody [1E8-H3-F11]

Anti-Hexokinase II antibody [1E8-H3-F11] used in Western Blot (WB). GTX49233
Anti-Hexokinase II antibody [1E8-H3-F11] used in Flow cytometry (FACS). GTX49233

Cat No. GTX49233

Host

Mouse

Clonality

Monoclonal

Clone Name

1E8-H3-F11

Isotype

IgG1

Application

WB, FACS

Reactivity

Human, Rat
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human Hexokinase II ( N-terminus) protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Hexokinase 2 , Hkii , Hxk2 , Hk2

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-Hexokinase II antibody [1E8-H3-F11] used in Western Blot (WB). GTX49233

GTX49233 WB Image

WB analysis of PC12, HeLa and Jurkat lysates using Hexokinase-2 antibody at a dilution of 1:1,000.

Anti-Hexokinase II antibody [1E8-H3-F11] used in Flow cytometry (FACS). GTX49233

GTX49233 FACS Image

FACS analysis of K562 cells using Hexokinase-2 antibody at a dilution of 1:100 (red) or an isotype control (blue).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf