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Hexokinase II antibody [4C5]

Overexpression
Anti-Hexokinase II antibody [4C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84357
Anti-Hexokinase II antibody [4C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84357
Anti-Hexokinase II antibody [4C5] used in Western Blot (WB). GTX84357
Anti-Hexokinase II antibody [4C5] used in Flow cytometry (FACS). GTX84357
Anti-Hexokinase II antibody [4C5] used in Western Blot (WB). GTX84357

Cat. No. GTX84357

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 4C5

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Dog
Package
100 μl ($539)
See all Hexokinase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase 2 , HKII , HXK2

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Hexokinase II antibody [4C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84357

GTX84357 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84357 Hexokinase II antibody [4C5].

Anti-Hexokinase II antibody [4C5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84357

GTX84357 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Hexokinase II plasmid using GTX84357 Hexokinase II antibody [4C5].

Anti-Hexokinase II antibody [4C5] used in Western Blot (WB). GTX84357

GTX84357 WB Image

Overexpression

WB analysis of HEK293T cells transfected with Hexokinase II plasmid (Right) or empty vector (Left) for 48 hrs using GTX84357 Hexokinase II antibody [4C5].
Loading : 5 ug per lane

Anti-Hexokinase II antibody [4C5] used in Flow cytometry (FACS). GTX84357

GTX84357 FACS Image

FACS analysis of HEK293T cells transfected with either Hexokinase II plasmid(Red) or empty vector control plasmid(Blue) using GTX84357 Hexokinase II antibody [4C5].

Anti-Hexokinase II antibody [4C5] used in Western Blot (WB). GTX84357

GTX84357 WB Image

WB analysis of various cell lines using GTX84357 Hexokinase II antibody [4C5].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 539