Hexokinase II antibody [4C5]

Cat. No. GTX84357

Host	Mouse
Clonality	Monoclonal
Clone Name	4C5
Isotype	IgG1
Application	WB, ICC/IF, FACS
Reactivity	Human, Dog
Package	100 μl ($539)

See all Hexokinase II products

Datasheet
Document

View product citations for antibody GTX84357 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
FACS	1:100

Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human HK2 (NP_000180) produced in HEK293T cell.

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase 2 , HKII , HXK2

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq]

Database

Gene ID: 3099 HK2
UniProt: P52789 HK2

Research Area

DATA IMAGES

GTX84357 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX84357 Hexokinase II antibody [4C5].

GTX84357 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with Hexokinase II plasmid using GTX84357 Hexokinase II antibody [4C5].

GTX84357 WB Image

WB analysis of HEK293T cells transfected with Hexokinase II plasmid (Right) or empty vector (Left) for 48 hrs using GTX84357 Hexokinase II antibody [4C5].
Loading : 5 ug per lane

GTX84357 FACS Image

FACS analysis of HEK293T cells transfected with either Hexokinase II plasmid(Red) or empty vector control plasmid(Blue) using GTX84357 Hexokinase II antibody [4C5].

GTX84357 WB Image

WB analysis of various cell lines using GTX84357 Hexokinase II antibody [4C5].
Loading : 35 ug per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 539

Qty

Hexokinase II antibody [4C5]

Cat. No. GTX84357

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84357 ICC/IF Image

GTX84357 ICC/IF Image

GTX84357 WB Image

GTX84357 FACS Image

GTX84357 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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All products are for research use only—Not for use in diagnostic or therapeutic applications.