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Hexokinase II antibody [HL2001]

Anti-Hexokinase II antibody [HL2001] used in Western Blot (WB). GTX637902
Anti-Hexokinase II antibody [HL2001] used in Western Blot (WB). GTX637902

Cat. No. GTX637902

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2001

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Product Note

This antibody is specific for human HK2 protein, and it does not cross react with human HK1, HK3, and HKDC1 protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human Hexokinase II.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase 2 , HKII , HXK2

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-Hexokinase II antibody [HL2001] used in Western Blot (WB). GTX637902

GTX637902 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody [HL2001] (GTX637902) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hexokinase II antibody [HL2001] used in Western Blot (WB). GTX637902

GTX637902 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody [HL2001] (GTX637902) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Available soon.
Custom Conjugations