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Hexokinase II antibody

KO/KD
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657
Anti-Hexokinase II antibody used in Western Blot (WB). GTX55657
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657
Anti-Hexokinase II antibody used in Western Blot (WB). GTX55657
Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657

Cat. No. GTX55657

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Hexokinase II products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human HK2 (NP_000180.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

hexokinase 2 , HKII , HXK2

Cellular Localization

Mitochondrion outer membrane

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657

GTX55657 IHC-P Image

IHC-P analysis of human colon tissue using GTX55657 Hexokinase II antibody.
Dilution : 1:200

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657

GTX55657 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX55657 Hexokinase II antibody.
Dilution : 1:200

Anti-Hexokinase II antibody used in Western Blot (WB). GTX55657

GTX55657 WB Image

WB analysis of various sample lysates using GTX55657 Hexokinase II antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657

GTX55657 IHC-P Image

IHC-P analysis of rat heart tissue using GTX55657 Hexokinase II antibody.
Dilution : 1:200

Anti-Hexokinase II antibody used in Western Blot (WB). GTX55657

GTX55657 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX55657 Hexokinase II antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Hexokinase II antibody used in IHC (Paraffin sections) (IHC-P). GTX55657

GTX55657 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX55657 Hexokinase II antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399