GeneTex
  • Country / Location Selection

United States (US)

Hexokinase III antibody [4F5]

Anti-Hexokinase III antibody [4F5] used in Western Blot (WB). GTX50057

Cat No. GTX50057

Host

Mouse

Clonality

Monoclonal

Clone Name

4F5

Isotype

IgG1

Application

WB, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

We recommend the following starting dilutions:
Western Blot: Use at 1:1,000 ~ 2,000.

Optimal working concentrations should be determined experimentally by the end user.

Calculated MW

99 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human Hexokinase III (1-923aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Hexokinase 3 , Hkiii , Hxk3 , Hk3

Background

Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase 3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase 3 may have anabolic functions, providing H6P for glycogen or lipid synthesis.

Database

Research Area

DATA IMAGES

Anti-Hexokinase III antibody [4F5] used in Western Blot (WB). GTX50057

GTX50057 WB Image

(A): Recombinant protein (20ng or 100ng) of Hexokinase four isoform were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Hexokinase 3 (1:1000). (B): Cell lysates (30?g) were resolved by SDS-PAGE, transferred to NC membrane and

REFERENCE

There are currently no references for Hexokinase III antibody [4F5] (GTX50057). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Hexokinase III antibody [4F5] (GTX50057). Be the first to share your experience with this product.
SDS
Sodium Azide.pdf