GeneTex
United States (US)

Hexokinase III antibody [C1C3]

GTX107822_40667_WB_19011507_322.jpg
GTX107822_40667_IHC_19011507_895.jpg
GTX107822_40667_20170621_IFA_19011507_568.jpg

Cat No. GTX107822

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

293T , A431 , HeLa

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Hexokinase III. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Hexokinase 3,Hkiii,Hxk3,Hk3

Background

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107822_40667_WB_19011507_322.jpg

GTX107822 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
5% SDS PAGE
GTX107822 diluted at 1:3000

GTX107822_40667_IHC_19011507_895.jpg

GTX107822 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Hexokinase III(GTX107822) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX107822_40667_20170621_IFA_19011507_568.jpg

GTX107822 ICC/IF Image

Hexokinase III antibody [C1C3] detects Hexokinase III protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Hexokinase III protein stained by Hexokinase III antibody [C1C3] (GTX107822) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169