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Hice1 (phospho Ser19/Ser20) antibody

Cat. No. GTX70011

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, ELISA

Reactivity

 Human
Package
100 μl ($399)
See all HICE1 products

APPLICATION

Application Note

Recommended Starting Dilutions:
For WB: Use at a dilution of 1:100
For ICC/IF: Use at a dilution of 1:50
Not yet tested in other applications. Optimal dilutions should be determined experimentally by the researcher.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa , K562 , Blocking peptide (GTX70011-PEP)

PROPERTIES

Form

Liquid

Buffer

1X PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic phosphopeptide (GTX70011-PEP) corresponding to the amino acids around Ser 19/20 conjugated to KLH

Purification

Affinity purified using antigen

Conjugation

Unconjugated

RRID

AB_11174659

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HAUS augmin like complex subunit 8 , DGT4 , HICE1 , NY-SAR-48

Cellular Localization

Cytoplasm

Background

HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).[supplied by OMIM, Jun 2010]

Database

Research Area

REFERENCE

Application Reference

Tsai CY et al. J Biol Chem 2011; 286 (34) : 30097-106 Aurora-A phosphorylates Augmin complex component Hice1 protein at an N-terminal serine/threonine cluster to modulate its microtubule binding activity during spindle assembly.
Application : WB, ICC/IF
Reactivity : Human
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SDS
PBS.pdf
Package List Price ($)
$ 399